Gram Rule Staining

Gram staining - Gram staining (or the Gram's method) is an empirical method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative) based on the chemical and physical properties of their cell walls.

Gram-negative - Gram-negative bacteria are those not stained dark blue or violet by Gram staining, in contrast to Gram-positive bacteria. On most Gram-stain preparations, Gram-negative organisms will be counterstained and appear red or pink.

Gram-positive - Gram-positive bacteria are those that are stained dark blue or violet by gram staining, in contrast to Gram-negative bacteria, which cannot retain the stain, instead taking up the counterstain and appearing red or pink. The stain is caused by a high amount of peptidoglycan in the cell wall, which typically, but not always lacks the secondary membrane and lipopolysaccharide layer found in Gram-negative bacteria.

Mycobacterium phlei - Mycobacterium phlei is a Gram-positive, acid fast bacteria of the genus Mycobacterium. They are named this way because they contain mycolic acids, which is also the reason that simple staining or Gram staining does not give good results with this microbe.

gramrulestaining

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